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M94A2776.TXT
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1994-10-25
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Document 2776
DOCN M94A2776
TI What is the real advantage of saliva collectors in anti-HIV testing?
DT 9412
AU Ubolyam S; Phanuphak P; Fang C; Department of Microbiology Chulalongkorn
Hospital, Bangkok,; Thailand.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):227 (abstract no. PB0339). Unique
Identifier : AIDSLINE ICA10/94369799
AB OBJECTIVE: To determine the advantage of the saliva collection device,
Omni-SAL in preserving saliva for anti-HIV testing as compared to saliva
collected by direct spitting. METHODS: Saliva specimens from 20 each of
HIV-infected and normal individuals were obtained by the saliva
collection device, Omni-SAL and by direct spitting into a plastic
container (Untreated saliva). Half of the specimen was kept at 4 degrees
C and the other half at 37 degrees C for 0, 1, 3, 7, 14 and 21 days. At
the end of each incubation period, aliquots were removed and stored at
-20 degrees C until simultaneously assayed for anti-HIV activity.
RESULTS: All Omni-SAL specimens from infected individuals tested
positive on Abbott HIV-1/2 EIA after storage either at 4 degrees C or 37
degrees C for up to 21 days. For untreated saliva, the anti-HIV activity
remained fully stable for 14 days at 4 degrees C but only for 7 days at
37 degrees C, 50% and 100% of the anti-HIV activity in the untreated
saliva was lost after 14 and 21 days of storage respectively. All saliva
specimens from 20 seronegative individuals were anti-HIV negative
irrespectively of the methods of collection or the duration and
condition of storage. DISCUSSION AND CONCLUSIONS: Saliva collection
device, Omni-SAL has the advantage over saliva collected by direct
spitting for anti-HIV testing in that it can preserve the anti-HIV
activity for up to 21 days at 37 degrees C storage whereas saliva
obtained by direct spitting is reliable for only 7 days at the same
condition.
DE Comparative Study Cryopreservation Evaluation Studies Human HIV
Antibodies/*ANALYSIS HIV Infections/*DIAGNOSIS Predictive Value of
Tests Preservation, Biological Saliva/*IMMUNOLOGY Salivary
Proteins/*ANALYSIS Specimen Handling/*INSTRUMENTATION Temperature
Time Factors MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).